Well, back in the mid 1990's I was adding 1-2ppm of Fe via TMG.
Not .2-.5ppm, that was 2-5x higher than PMDD suggested at the time.
I was a bit more conservative because I'd not yet tested it myself and could not say.
I speculated, but that's easy to do.
I needed more evidence.
To test for traces:
You need to have everything else non limiting.
If you do not this will confound your test results and be impossible to tease any useful conclusions out of/or answer your original question.
From there you can go two main directions: low to high, or high to low.
Now if you start at limiting levels, you can stunt the plants, this might take time to recover.
Bad idea.
So add excess, then slowly back off.
Do this in 3 to 4 week intervals for each treatment/Test mls per tank size.
So if a tank is 75 liters, try adding 5mls a day and then after 4weeks, lower it to 4, then 4 more weeks, try 3mls for 4 weeks, then 2mls and 1 ml etc.
You need at least 3 weeks to see a difference due to plant's reserves.
They horde trace nutrients and you need to depelete to see any negative plant health related signs.
During this test, you need to be sure the CO2 and other nutrients are very well maintained.
I suggest 2x a week 50% water changes when you do this and dose thereafter or go to daily dosing routines.
Keep up on things, otherwise all your work will be wasted
Done that a few times myself.
Grrrrrrrrrrrrrrrrrrr
So the only true method I really know of beside measuring dry weight differences or O2 level differences etc, is simly watching the plant's responses over time when you isolate the trace dosing.
Basically, you use the plants as the indicator for plant health, which is really what we want to know, not some PPM level or test kit reading that we have to infer plant health to.
Regards,
Tom Barr